Three subtypes expressions of leptin receptor in the rat anterior pituitary and influence of leptin on intracellular free Ca2+ of the rat growth hormone cell.
- Author:
Ya-Li LIU
1
;
Yan-Qing ZHONG
;
Yun-Long ZHU
;
Su-Min CHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Cells, Cultured; Growth Hormone; metabolism; Male; Pituitary Gland; metabolism; Rats; Rats, Sprague-Dawley; Receptors, Cell Surface; metabolism; Receptors, Leptin; metabolism
- From: Chinese Journal of Applied Physiology 2004;20(4):349-353
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the expression of Leptin receptors (OB-R) in male rat anterior pituitary, and study the influence of Leptin on the level of intracellular free Ca2+ ([Ca2+]i) in the cultured growth hormone (GH) cell of male rat pituitary.
METHODSRT-PCR method was used to observe the expression of Leptin receptors (OB-R) in male rat anterior pituitary. We used grade centrifuging method to get growth hormone (GH) cell, and [Ca2+]i in GH cell was examined by laser scanning confocal system.
RESULTSOB-R mRNA were expressed in male rat anterior pituitary, including OB-R (common form), OB-Ra (short form) and OB-Rb (long form). There were about 70% or 80% GH cell by grade centrifuging. Leptin at 10(-8)mol/L could decrease the level intracellular free Ca2+ ([Ca2+]i) in cultured GH cell.
CONCLUSIONThere are three subtypes of Leptin receptors expressions in male rat anterior pituitary, and Leptin could reduce intracellular free Ca2+ level of GH cell markedly.
