Effect of ginkgolides on gene expression of HIF-1alpha in primary cultured neurons.
- Author:
Li ZHU
1
;
Xiao-Mei WU
;
Jian-Cheng LI
;
Shu-Yi JIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Hypoxia; Gene Expression; Ginkgolides; pharmacology; Hypoxia-Inducible Factor 1, alpha Subunit; genetics; metabolism; Mice; Mice, Inbred ICR; Neurons; drug effects; metabolism; RNA, Messenger; genetics
- From: Chinese Journal of Applied Physiology 2004;20(4):354-357
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of ginkgolides (Gin) on the expression of hypoxia inducible factor-1 (HIF-1alpha) in hypoxic/ischemic neurons.
METHODSThe gene expression of HIF-1alpha pretreated with or without Gin (37.5 microg/ml) was observed by RT-PCR on primary cultured cortical neurons in the condition of hypoxia and oxygen-glucose deprivation.
RESULTSSome basic expression of HIF-1alpha mRNA were observed in cultured cortical neurons. The expression of HIF-1alpha mRNA increased after 24 h treatment with Gin. The level of HIF-la mRNA increased also after 1 h hypoxia and further enhanced after the pretreatment with Gin. The expression of HIF-1alpha mRNA decreased with the deprivation of both oxygen and glucose, which reversed after the pretreatment of Gin.
CONCLUSIONGin could increase the expression of HIF-1alpha mRNA in hypoxic/ischemic cortical neurons.
