Changes of HO-1 genes expression in liver following ischemia/reperfusion of limbs and their significance in rats.
- Author:
Zhong-Li SHI
1
;
Yi-Ling LING
;
Yi-Qun LING
;
Yu-Xia YAO
;
Jun-Lin ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Gene Expression; Heme Oxygenase (Decyclizing); genetics; metabolism; Hindlimb; blood supply; Liver; metabolism; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Reperfusion Injury; metabolism
- From: Chinese Journal of Applied Physiology 2004;20(4):367-370
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo detect the changes of inducible heme oxygenase (HO-1) expression in liver following ischemia/reperfusion (I/R) of hindlimbs and to elucidate their significance.
METHODSI/R was established using the occlusion of the femoral arteries for 4h and reopening for 2-24 h in rats. The expression of HO-1 mRNA and HO-1 protein in liver tissue were detected with reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical technique, respectively. The observation of pathologic changes of liver was made following the inhibition of HO-1 by zinc protoporphyrin (ZnPP).
RESULTSCompared with control groups, the relative expression level of HO-1 mRNA significantly increased in I/R group. There were more HO-1 positive hepatocytes in I/R group than control groups. The pathologic changes of liver tissue became more severe in I/R + ZnPP group.
CONCLUSIONThe expressions of HO-1 mRNA and protein in liver tissue are significantly upregulated, induction of HO-1 is involved in protection for hepatocytes during the I/R of hindlimbs.
