Effect of Safflor yellow against neonatal hypoxia on expression of NOSs mRNA.
- Author:
Dong-Mei YIN
1
;
Shu-Yi JIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; Chalcone; analogs & derivatives; pharmacology; therapeutic use; Gene Expression; Hypoxia; metabolism; prevention & control; Nitric Oxide Synthase Type I; metabolism; Nitric Oxide Synthase Type II; metabolism; Nitric Oxide Synthase Type III; metabolism; Quinones; pharmacology; therapeutic use; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2004;20(4):371-374
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of Safflor yellow on the gene expression of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in neonatal asphyxia.
METHODS30 minutes after SY 7 g/kg weight intraperitoneally was administered on the neonatal rats. After asphyxia for 40 minutes,the neonatal rats were reoxygenated for 48 h, and the nitric oxide synthases (NOSs) mRNA expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction.
RESULTSNeuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) were up in hypoxia/reoxygenation (H/R) 48 h group, while both of them were down significantly in SY group, but no change was observed on endothelial nitric oxide synthase (eNOS).
CONCLUSIONThe protective of SY from brain damage induced by neonatal asphyxia might be associated with expression of NOSs mRNA.
