Measurement of systolic/diastolic function in isolated ventricular myocytes by video-based motion edge-detection system.
- Author:
Hai-Feng ZHANG
1
;
Bo ZHANG
;
Xiao-Dong ZHANG
;
Feng GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Cells, Cultured; Cytological Techniques; Heart Ventricles; cytology; Myocardial Contraction; Myocytes, Cardiac; physiology; Rats; Rats, Sprague-Dawley; Single-Cell Analysis; Video Recording; instrumentation
- From: Chinese Journal of Applied Physiology 2004;20(4):410-414
- CountryChina
- Language:Chinese
-
Abstract:
AIMSingle cardiomyocytes were isolated from adult rat hearts and myocyte contraction was recorded by a video-based motion edge-detection system.
METHODSSingle ventricular myocytes were enzymatically isolated, loaded with fura-2/AM (0.5 micromol/L) for 30 min in dark at room temperature and field-stimulated (0.5 Hz, 5 ms), and both myocyte contraction and intracellular fluorescence intensity were simultaneously assessed by a video-based motion edge-detection system.
RESULTSUsing the video-based motion edge-detection system, one may real-time beat-to-beat observe and record the amplitude, duration and velocity of both myocyte shortening/ relengthening and intracellular calcium transient, and then directly describe the change of isolated ventricular myocyte mechanics.
CONCLUSIONDirect measurement of isolated ventricular myocyte mechanics using the video-based motion edge-detection system is an increasingly important technique in cardiac physiology that provides fundamental information on myogenic and excitation-contraction coupling of the heart in drug intervention and gene manipulation.
