The state of SHP-1 and CD45 in lymphocyte under high partial pressure of oxygen.
- Author:
Wei-gang XU
1
;
Heng-yi TAO
;
Yun LIU
;
Chun-lei JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Leukocyte Common Antigens; metabolism; Lymphocytes; enzymology; metabolism; Male; Oxygen; metabolism; Partial Pressure; Phosphorylation; Protein Tyrosine Phosphatase, Non-Receptor Type 6; metabolism; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; metabolism
- From: Chinese Journal of Applied Physiology 2005;21(1):15-18
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore the function state of protein tyrosine phosphatase (PTP) SHP-1 and CD45 under high partial pressure of oxygen (Po2) in lymphocyte.
METHODSPrimarily cultured rat splenic lymphocytes were treated with different pressure-duration of oxygen that led to promotion or inhibition of cell function. The catalytic activities, protein expression and tyrosine phosphorylation of SHP-1 and CD45 were determined.
RESULTSThe activity of SHP-1 was decreased after high Po2 treatment no matter what pressure-duration was, while the activity of CD45 was decreased only after high PoF that led to inhibition of lymphocyte function. The proteins expressed and tyrosine phosphorylation levels of two enzymes had no alteration in most treatment groups.
CONCLUSIONThe decrease of PTP catalytic activities might be caused by their structures modification by high Po2 induced reactive oxygen species. SHP-1 and CD45 might be one of the key points of action through which high PO2 exerts its effects on lymphocytes.
