Effect of propofol on anti-oxidation capability in the ischemia/reperfusion injury after hepatic ischemia in rabbits.
- Author:
Wan-tie WANG
1
;
Li-na LIN
;
Wei WANG
;
Dong LI
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; metabolism; Animals; Free Radical Scavengers; metabolism; Liver; blood supply; ultrastructure; Malondialdehyde; metabolism; Oxidation-Reduction; drug effects; Propofol; pharmacology; therapeutic use; Rabbits; Reactive Oxygen Species; metabolism; Reperfusion Injury; drug therapy; metabolism; Superoxide Dismutase; metabolism; Xanthine Oxidase; metabolism
- From: Chinese Journal of Applied Physiology 2005;21(1):26-29
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the role of oxygen free radicals (OFR) in hepatic ischemia and reperfusion injury (HI/RI) and effect of propofol on them.
METHODSThe rabbits were randomly divided into three groups (n=10), sham operated group (Control), HIR group(I/R) and HIR + propofol group (PRO). Changes of several parameters which included malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XO) and alanine aminotransferase(ALT) were measured before ischemia, 45 minutes after ischemia and 45 minutes after reperfusion in plasma. Meanwhile MDA concentration, SOD, XO activities and ALT value in liver tissue were measured, and the ultrastructure changes in liver tissue were observed under electron microscope at 45 minutes after reperfusion.
RESULTSAs compared with group control, XO, MDA and ALT increased and SOD decreased during HI/RI (P < 0.05 and P < 0.01) in plasma, and XO, MDA increased as well as SOD decreased at 45 minutes after reperfusion (P < 0.05 and P < 0.01) in liver tissue and there were abnormal changes of the hepatic ultrastructure at 45 minutes after reperfusion. Propofol reversed the results of mentioned indices as above markedly (P < 0.05 and P < 0.01).
CONCLUSIONOFR is an important factor during HI/RI, propofol may attenuate hepatic ischemia-reperfusion injury by dropping OFR level (raising SOD activity and dropping XO activity) and antagonizing lipid pe-reoxidation (reducing MDA content).
