The effect of NO, ET-1 on brain injury after hind limbs ischemia/reperfusion in rats.
- Author:
Yong-qi HU
1
;
Lian-yuan ZHANG
;
Hong-jie LI
;
Jun PENG
;
Shu-yun DONG
;
Xiu-li MEN
;
Quan-hui YANG
;
Hong-xia ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Injuries; pathology; Endothelin-1; metabolism; Extremities; blood supply; Male; Nitric Oxide; metabolism; Rats; Rats, Wistar; Reperfusion Injury; metabolism; pathology
- From:Chinese Journal of Applied Physiology 2005;21(1):30-33
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the roles of nitric oxide (NO) and ET-1 in brain injury after hind limbs ischemia/reperfusion in rats and to investigate the effect of NO/ ET-1 balance on brain injury.
METHODSOn a model of the hind limbs ischemia/reperfusion (LI/R) of rats, we used L-Arg(L-arginine, L-Arg), one of the substrates in the process of nitric oxide, aminoguanidine (AG) which inhibits nitric oxide synthase(NOS) and ETA receptor antagonist BQ123, to observe the changes of NO, ET-1, MDA, XOD, SOD, LDH in plasma and tNOS, iNOS, cNOS, NO, ET-1, MDA, XOD, MPO, SOD in brain tissue.
RESULTSCompared with the control group, the content of MDA, XOD, LDH in plasma and MDA, XOD, MPO in brain tissue increased. The activity of SOD decreased (P < 0.01). The content of tNOS, iNOS in brain tissue increased, cNOS decreased (P < 0.01). The content of NO, ET-1 in I/R group in plasma and brain tissue increased, the ratio of NO/ET-1 decreased. The brain injury was deteriorated. After using L-Arg and BQ123, the ratio of NO/ET-1 in plasma and brain tissue increased, the brain injury lightened. Whereas after using AG, the ratio of NO/ET-1 decreased, brain injury became more serious.
CONCLUSIONThe NO/ET-1 ratio decreased after LI/R, brain injury became more serious.
