Effect of NOS and PTEN on cardiomyocyte hypertrophy induced by angiotensin II.
- Author:
Yang WU
1
;
Ya-e HU
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin II; pharmacology; Animals; Animals, Newborn; Cardiomegaly; chemically induced; metabolism; pathology; Cells, Cultured; Myocytes, Cardiac; drug effects; metabolism; pathology; Nitric Oxide Synthase Type II; metabolism; Nitric Oxide Synthase Type III; metabolism; PTEN Phosphohydrolase; metabolism; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2005;21(1):41-45
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of NOS and PTEN on the hypertrophic response induced by angiotensin II in the primary culture of neonatal rat cardiomyocytes.
METHODSTotal protein content of cardiomyocytes was used as the index of cardiac myocyte hypertrophy. eNOS mRNA, iNOS mRNA and PTEN mRNA expression were assessed using RT-PCR normalized with GAPDH. PTEN protein was determined by Western blot and immunohistochemistry method.
RESULTS(1) On day 1 after Ang II treatment, the expression of eNOS mRNA was significantly decreased whereas iNOS mRNA expression was significantly increased. The effect of Ang II on NOS expression was inhibited by L-arginine. (2) Total protein content of cardiomyocytes increased significantly on day 5 after Ang II treatment, and PTEN protein expression was significantly decreased. The increased protein content and the decreased expression of PTEN protein were inhibited by L-Arg. The L-arginine effect was blocked by L-NAME(NOS inhibitor). (3) The positive immunocytochemical product of PTEN was mainly located in the nucleus of myocardiocyte.
CONCLUSIONThese results indicate that NOS and PTEN may take part in the process of cardiac myocyte hypertrophy induced by Ang II. The effect of L-arginine on cardiomyocytes may be mediated by NOS/NO and PTEN.
