Regulatory effects of nucleotides on ATP-sensitive potassium channel opener pinacidil-induced vascular relaxation.
- Author:
Hua-mei HE
1
;
Chao-liang LONG
;
Hai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta; cytology; drug effects; Glyburide; pharmacology; In Vitro Techniques; KATP Channels; metabolism; Male; Muscle, Smooth, Vascular; drug effects; Nucleotides; pharmacology; Pinacidil; pharmacology; Rats; Rats, Wistar; Vasodilator Agents; pharmacology
- From: Chinese Journal of Applied Physiology 2005;21(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
AIMIn order to evaluate the regulatory effects of nucleotides and adenosine on ATP-sensitive potassium channel (K(ATP)) in artery smooth muscles, the effects of them on vascular relaxation induced by K(ATP) opener pinacidil(Pin) were investigated.
METHODSThe isolated endothelium- denuded aorta rings were preincubated with nucleotides or nucleotides and glibenclamide (Gli) for 10 min, the vascular relaxation induced by Pin in aorta precontracted with 20 mmol x L(-1) KCl was observed.
RESULTSAfter the isolated endothelium-denuded aorta rings were preincubated with ATP, ADP, UDP, GTP and adenosine (Ade) 100 micromol x L(-1) respectively, the vascular relaxation induced by Pin was changed as following: (1) ATP could inhibit the K(ATP) activation by Pin and enhance the blockade of K(ATP) by Gli. (2) ADP could inhibit the K(ATP) activation by Pin and attenuate the blockade of K(ATP) by Gli. (3) The regulatory effect of Ade on K(ATP) was similar with that of ADP. (4) UDP could enhance the K(ATP) activation by Pin and attenuate the blockade of K(ATP) by Gli. (5) GTP could enhance the K(ATP) activation by Pin, but had no effects on the blockade of K(ATP) by Gli.
CONCLUSIONNucleotides and adenosine, related to energy metabolism, could modulate the functions of K(ATP) in vascular smooth muscle. But their pharmacological characteristics were different.
