Effect of anti-human VEGF monoclonal antibody on VEGF level in serum of nude mice transplanted buccal carcinoma: a quantitative study.
- Author:
Si-lian FANG
1
;
Da-zhang WANG
;
Jing-yi ZHANG
;
Guang-yong ZHENG
;
Xi-chuan YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; pharmacology; Biomarkers, Tumor; blood; Carcinoma; blood; Humans; Mice, Inbred BALB C; Mice, Nude; Mouth Mucosa; pathology; Neoplasm Transplantation; Neoplasms; blood; Vascular Endothelial Growth Factor A; blood
- From: West China Journal of Stomatology 2004;22(4):320-322
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEQuantitative study of the effect of anti-human VEGF mAb E11 to VEGF level in serum of nude mice transplanted buccal carcinoma.
METHODSE11 was administered into BALB/c nu/nu mice which were transplanted human buccal carcinoma. The saline was administrated as negative control. Mice were killed at 18 days. The VEGF level in serum of mice was determined by improved indirect ELISA.
RESULTSCompared with the VEGF level in serum of mice in saline group, it was dramatically decreased in E11 group. The VEGF level in serum of mice treated E11 by subcutaneous was lowest and only reached (1.17 +/- 0.13) microg/L.
CONCLUSIONIt demonstrated that the anti-human VEGF mAb could reduce the VEGF level in serum by binding VEGF, and block its biological activity. It indicates that VEGF in serum of malignant tumor patient is a new tumor marker.
