The effects of growth hormone on rabbit's mandibular condylar chondrocytes proliferation and secretion in vitro.
- Author:
Ning HUANG
1
;
Song-jiao LUO
;
Hong-mei YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cartilage, Articular; cytology; Cell Proliferation; drug effects; Cells, Cultured; Chondrocytes; drug effects; secretion; Collagen Type II; drug effects; Growth Hormone; pharmacology; Mandibular Condyle; cytology; Rabbits
- From: West China Journal of Stomatology 2004;22(5):370-372
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of growth hormone (GH) on the proliferation and type II collagen secretion of chondrocytes of mandibular condyle in rabbit in vitro.
METHODSFlow cytometry (FCM) and immunohistochemical technique were employed to observe the possible changes.
RESULTS(1) The exogenic GH can enhance the proliferation and synthesis of DNA of the chondrocytes of mandibular condyle in rabbit in vitro. The suitable concentration of GH is 10 microg/ml. The synthesis of DNA reaches the highest level after 12 hours, while the proliferation index (PI) hits the highest after 24 hours. (2) GH (10 microg/ml) can stimulate the secretion of type II collagen of the chondrocytes.
CONCLUSIONThe exogenic GH can enhance the proliferation, the synthesis of DNA and the secretion of type II collagen of the chondrocytes of mandibular condyle in rabbit in vitro.
