Studies on the transfection of umbilical endothelia with catalytic subunit of telomerase.
- Author:
Xiao-ming DAI
1
;
Long-jiang LI
;
Yu-ming WEN
;
Chang-mei WANG
;
Hua LIU
;
Kun LIU
;
Sheng-fu LI
Author Information
- Publication Type:Journal Article
- MeSH: Catalytic Domain; genetics; Cells, Cultured; Endothelium, Vascular; cytology; Humans; Telomerase; genetics; Transfection; Umbilical Veins; cytology
- From: West China Journal of Stomatology 2004;22(5):373-389
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the variety of proliferating ability of umbilical endothelia (UE) transfected by plasmid pBABE-HYGR-hTERT.
METHODSUE was identified from two aspects: morphology and CD34 labeling technique. The plasmid was obtained and identified by alkali splitting and gel electrophoresis. Liposomes were used to transfect UE. RT-PCR based telomeric repeat amplification protocol (TRAP) assay was used to measure the telomerase activity of endothelia.
RESULTSUE arranged as "cobblestone" and were positive of CD34 labeling. Endothelia transfected by pBABE-HYGR-hTERT(HC) had an raised absorbance of 0.889. The shape of growth curve of HC was similar to UE. But the absorbance of MTT test and the amount of HC were prior to UE at every measuring time and the amount of HC increased four times within 8 days (P < 0.05).
CONCLUSIONThe transfection of pBABE-HYGRO-hTERT had greatly improved the proliferating abilities and activated the telomerase of UE.
