Recombinant human bone morphogenetic protein-2 transform NIH3T3 fibroblast to osteo-like cells.
- Author:
Jun-lin WANG
1
;
Yuan LIU
;
Yan JIN
;
Xin-wen WANG
;
Yuan LI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; drug effects; metabolism; Animals; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; pharmacology; Cell Proliferation; drug effects; DNA; drug effects; Fibroblasts; cytology; Mice; NIH 3T3 Cells; Osteocalcin; drug effects; Recombinant Proteins; pharmacology; Transforming Growth Factor beta; pharmacology
- From: West China Journal of Stomatology 2004;22(5):420-422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on NIH3T3 cells.
METHODSThe NIH3T3 cells were cultured in DMEM with 50 microg/L rhBMP-2. The proliferation ability, alkaline phosphatase (ALP) activity and osteocalcin (OC) quantity of NIH3T3 cells were investigated.
RESULTSThe cells showed decreased proliferation ability but enhanced ALP activity and OC quantity.
CONCLUSION50 microg/L rhBMP-2 can transform NIH3T3 fibroblasts to osteo-like cells.
