Expression related to vascular endothelial growth factor C and induced nitride oxide synthesizase in lymph node micrometastasis of oral squamous cell carcinoma.
- Author:
Zhi-jian XIE
1
;
Xiao-feng YANG
;
Qiu-liang WU
;
Zhi-yuan GU
;
Yin-kai ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; metabolism; pathology; Humans; Keratins; metabolism; Lymphatic Metastasis; Mouth Neoplasms; metabolism; pathology; Neoplasm Micrometastasis; Nitric Oxide Synthase Type II; metabolism; Vascular Endothelial Growth Factor C; metabolism
- From: West China Journal of Stomatology 2004;22(6):445-459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship of vascular endothelial growth factor C (VEGF-C) and induced nitride oxide synthesizase (iNOS) expression in lymph node micrometastasis of oral squamous cell carcinoma.
METHODSSamples were obtained from 47 cases of oral squamous cell carcinoma and 15 cases with normal oral mucosa, VEGF-C and iNOS mRNA expression were detected by RT-PCR method. Lymph node micrometastasis of 10 normal lymph nodes and 355 lymph nodes from 47 cases of oral squamous cell carcinoma was detected with immunohistochemical reaction in cytokeratin antibody.
RESULTSThe percentages in tumors with higher expression were 57.4% for VEGF-C, 68.1% for iNOS (P < 0.05). They were significantly higher than that of normal groups. Significant positive relationship was found between VEGF-C and iNOS (P < 0.01). The positive rate of cytokeratin (CK) was 48.9%. Significant positive relationship was found between VEGF-C and CK, iNOS and CK (P < 0.01). The expression rates of CK in positive group of VEGF-C and iNOS were 63.0%, 65.6% respectively, and were significant higher than negative groups.
CONCLUSIONExpression of VEGF-C and iNOS in lymph node micrometastasis of oral squamous cell carcinoma is significant related.
