The positive effect of transforming growth factor beta on ectomesenchymal stem cells of embryonic facial processes differentiating to smooth muscle cells.
- Author:
Man-jing DENG
1
;
Yan JIN
;
Jun-nan SHI
;
Yuan LIU
;
Yuan LI
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Cell Differentiation; drug effects; Cells, Cultured; Humans; Mesenchymal Stromal Cells; cytology; drug effects; Myocytes, Smooth Muscle; cytology; drug effects; Transforming Growth Factor beta; pharmacology
- From: West China Journal of Stomatology 2004;22(6):460-462
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of transforming growth factor beta (TGF-beta) on ectomesenchymal stem cells differentiating to smooth muscle cells.
METHODS60 pmol/L TGF-beta was added to the ectomesenchymal stem cells of embryonic facial processes. Immunohistochemistry assay and image analysis were used to value the expression extent of a smooth muscle actin (alpha-SMA) and quantitative RT-PCR was used to value the quantity of alpha-SMA.
RESULTS2 days later, about 95% cells in TGF-beta group and 65% cells in control group without differentiation inhibitor expressed alpha-SMA. Expression of alpha-SMA in TGF-beta group was stronger than that of control group after one and two days. Quantitative RT-PCR showed the quantity of alpha-SMA mRNA in treated group cells was more than that of in control group.
CONCLUSIONQuantity of alpha-SMA in TGF-beta group is more than that of spontaneous differentiation group. TGF-beta has positive effect on ectomesenchymal stem cells differentiating to smooth muscle cells.
