The effects of HSV-tk suicide gene and wild-type p53 gene on pleomorphic adenoma cells of salivary gland.
- Author:
Xu WANG
1
;
Jie WANG
;
Fu-sheng DONG
;
Yu-ying DONG
;
Ya-li HOU
Author Information
- Publication Type:Journal Article
- MeSH: Adenoma, Pleomorphic; Antiviral Agents; Cell Line, Tumor; Cell Survival; Ganciclovir; Genes, p53; Genetic Therapy; Genetic Vectors; Humans; Salivary Glands; Simplexvirus; Thymidine Kinase; Transfection
- From: West China Journal of Stomatology 2005;23(1):65-68
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the therapeutic effects of combined gene therapy of wild type p53 (wt-p53) and herpes simplex virus thymidine kinase (HSV-tk) gene on pleomorphic adenoma cells of salivary gland.
METHODSWild type p53 and HSV-tk gene were transfected into human pleomorphic adenoma cells of salivary gland by using recombinant adenovirus vector. The efficiency of transfection was checked and gene was expressed by RT-PCR methods. The cell inhibition after transfected was verified by light microscope and MTT.
RESULTSThe proliferation of the pleomorphic adenoma cells transfected wt-p53 and HSV-tk gene was inhibited and the cell survival rate decreased to 54% and 38% respectively in 5 days. However, when wt-p53 gene combined with HSV-tk/GCV system, the killing effects was significantly stronger (P < 0.05) and the cell survival rate decreased to 20%.
CONCLUSIONCombining p53 gene with HSV-tk/GCV system for gene therapy in pleomorphic adenoma cells of salivary gland is a valuable method.
