Biological effects of nano-hydroxyapatite/polyamide 66 on the dental pulp cells.

Author: Qin SU ¹ ; Ling YE ; Xue-dong ZHOU
Author Information

1. Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Publication Type:Journal Article
MeSH: Biocompatible Materials; Cell Line; Cell Proliferation; Cells, Cultured; Dental Pulp; Durapatite; Humans; Nylons
From: West China Journal of Stomatology 2005;23(1):79-81
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the biological effects of the new nano-hydroxyapatite/polyamide 66 biological composites (nHA-PA66) on the dental pulp cells.
METHODSAfter interaction with the nHA-PA66 eluate, the growth, proliferation, and function of the in vitro cultured human dental pulp cells were studied by cell culture technique, inverted phase-contrast microscope observation, MTT assay, flow cytometry, ALP activity assay and quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) analysis.
RESULTSThe cultured pulp cells grew well and showed no morphological variation. Moreover, this material had no negative effects on the proliferation, cell cycle, ALP activity and the expression of dentin sialophosphoprotein (DSPP) mRNA of the pulp cells.
CONCLUSIONAs a new nano-biomaterial, nHA-PA66 has good biocompatibility to the pulp cells, but no obvious bioactivity.