The study of cell biocompatibility of new pattern biphasic calcium phosphate nanocomposite in vitro.
- Author:
Tao WANG
1
;
Wei-dong TIAN
;
Lei LIU
;
Xi-zhe CHENG
;
Yun-mao LIAO
;
Sheng-wei LI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Animals; Bone Marrow Cells; Bone and Bones; Cell Adhesion; Durapatite; Hydroxyapatites; Materials Testing; Nanocomposites; Osteoblasts; Osteocalcin; Rats; Tissue Engineering; Tissue Scaffolds
- From: West China Journal of Stomatology 2005;23(2):106-109
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the cell biocompatibility of porous biphasic calcium phosphate nanocomposite in vitro.
METHODSBone marrow mesenchymal cell (BMSCs) obtained from SD rat bone marrow were in vitro induced and proliferated. Afler their osteoblast phenotypes were verified, BMSCs were seeded onto prepared porous biphasic calcium phosphate nanocomposite (Experiment group) and common porous hydroxyapatite (Control group). The cell adhesion was evaluated by scanning electron microscope. Synthesis of alkaline phosphatase enzyme (ALP) and osteocalcin were detected and cell cycle was detected by flow cytometry.
RESULTSBMSCs could fully attach to and extend on the material in experiment and control group, Moreover, experiment group were superior to control group in adhesion, proliferative abilities and osteogenic activity.
CONCLUSIONBMSCs can differentiate to osteoblast phenotype; the porous biphasic calcium phosphate nanocomposite as bone tissue engineering scaffold has good cell biocompatibility.
