A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel.
- Author:
Cheng-Biao ZHANG
1
;
Xiao-Lu YU
;
Zhi-Qiang PAN
;
Cui-Jie SHAO
;
Jun-Li CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Separation; methods; Female; Ganglia, Spinal; cytology; Ion Channel Gating; physiology; Ion Channels; Male; Neurons; cytology; physiology; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2011;27(4):510-512
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.
METHODSDRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.
RESULTSThe acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.
CONCLUSIONThe acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.
