Effect of TLR4 on the migration of asthmatic airway smooth muscle cells induced by airway epithelial cells.
- Author:
Hai-Ying SU
1
;
Bi-Wen MO
;
Jiang-Hong WEI
;
Jian-Wei HUANG
;
Chang-Ming WANG
;
Jin-Rong ZENG
;
Qing XU
;
Yun LIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Asthma; metabolism; Cell Movement; Cells, Cultured; Chemokine CCL5; metabolism; Epithelial Cells; metabolism; Interleukin-8; metabolism; Myocytes, Smooth Muscle; cytology; metabolism; Rats; Rats, Sprague-Dawley; Toll-Like Receptor 4; metabolism; Tumor Necrosis Factor-alpha; pharmacology
- From: Chinese Journal of Applied Physiology 2012;28(2):103-106
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of Toll-like receptor 4 (TLR4) activation on the migration of asthmatic airway smooth muscle cell (ASMCs) induced by airway epithelial cells.
METHODSPrimary ASMCs were cultured by the method of cell digestion. Cell culture supernatant of RTE cells were collected by TNF-alpha stimulation of epithelial cells. Detected the IL-8 and RANTES levels in the supernatant. The transmembrane migration of asthmatic ASMCs were detected by Modified Boyden chemotaxis chamber. The effect of TLR4 on the migration of asthmatic ASMCs induced by epithelial cells with TLR4 antibody drugs as a tool.
RESULTSThe levels of IL-8 and RANTES in the supernatant of TNF-alpha groups were significantly increased, and that in the 20 ng/ml group was significantly higher than other groups (P < 0.01). The transmembrane migration of asthmatic ASMCs groups was increased than that of control group. The transmembrane migration of asthmatic ASMCs from asthma group and TNF-alpha + TLR4 antibody group was significantly decreased compared with that in TNF-alpha group (P < 0.01). The migration of asthma ASMCs from TNF-alpha + TLR4 antibody group was increased than that of asthma group (P < 0.05).
CONCLUSIONTLR4 in the surface of asthmatic ASMCs may be activated by cytokines secreted by the airway epithelial cells and enhance the transmembrane migration of asthmatic ASMCs induced by airway epithelial cells so that it plays a role in airway remodeling of asthma.
