Protective effects of SUR2B/Kir6.1 potassium channel opener natakalim against RAVECs injuries induced by hypoxia.
- Author:
Hua WEI
1
;
Yan-qing CAO
;
Jing-dan ZHANG
;
Zhe PENG
;
Dan-feng YANG
;
Jia-yin LIU
;
Yong-ping SHI
;
Yong-lin YANG
;
Hai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Allyl Compounds; pharmacology; Animals; Aorta; cytology; metabolism; Cell Hypoxia; Cells, Cultured; Endothelial Cells; metabolism; Endothelin-1; metabolism; Intercellular Adhesion Molecule-1; metabolism; Male; Propylamines; pharmacology; RNA, Messenger; genetics; Rats; Rats, Wistar; Vascular Endothelial Growth Factor A; metabolism; Vascular System Injuries; metabolism
- From: Chinese Journal of Applied Physiology 2012;28(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effects of natakalim against rat aortic vascular endothelial cells (RAVECs) injuries induced by hypoxia and its mechanisms.
METHODSSelecting RAVECs as a cell model injured by hypoxia, these RAVECs were divided into 5 groups: i.e. control group, hypoxia group, natakalim low, medium and high group. The cell survival rate was determined by MTT assay, con was measured using Griess Assay, RT-PCR was used to examine t he expression of intercellular adhesion molecule-1 (ICAM-1), vascular endothelial growth factor (VEGF), endothelin-1 (ET-1) mRNA in RAVEC.
RESULTSNatakalim could reverse hypoxia-induced changes in endothelial cell function, including increased endothelial cell survival rate and level of NO concentration, significantly inhibited the hypoxia-induced endothelial ICAM-1, ET-1, VEGF mRNA expression levels increased.
CONCLUSIONNatakalim have protective effects on hypoxia-induced changes in endothelial cell function, increasing of permeation, excess expression of cell adhesion molecules.
