Construction of the skeletal muscle-specific TbetaR II knockout mice.
- Author:
Xu WANG
1
;
Jing WANG
;
Lu WANG
;
Hua-min WANG
;
You-fei GUAN
;
Ming FAN
;
Xiao-ping CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Mice; Mice, Knockout; Mice, Transgenic; Muscle, Skeletal; metabolism; Protein-Serine-Threonine Kinases; genetics; metabolism; Receptors, Transforming Growth Factor beta; genetics; metabolism; Recombination, Genetic; Signal Transduction
- From: Chinese Journal of Applied Physiology 2012;28(3):284-287
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo generate the skeletal muscle-specific transforming growth factor beta receptor II (TbetaR II) gene knockout mice for the research on the function of the TbetaR II gene in skeletal muscles.
METHODSTbetaR II (flox/flox) mice were generated using embryonic stem cell technology. The MCK-Cre mice were engineered containing Cre recombinase under the control of creatine kinase (MCK) muscle-specific promoter. TbetaR II (flox/flox) mice were crossed with MCK-Cre mice generating TbetaR II (flox/flox)/MCK-Cre double Tg mice. And then, TbetaR II (flox/wt) /MCK-Cre(+) double Tg mice were crossed with TbetaR II (flox/flox) mice to generate TbetaR II (flox/flox)/MCK- Cre(+) mice genetically ablating TbetaR II in cre-expressing skeletal muscle cells.
RESULTSAs predicted, mice lacking TbetaR II by gene targeting in skeletal muscles were generated first in the world using Cre/loxP system. TbetaR II null mutant mice were viable, fertile and showed apparently normal development.