The effect of Hsp72 on IL-6, IL-8 expression and activation of NF-kappaB in synoviocytes of rheumatoid arthritis.
- Author:
Xin-Jing LUO
1
;
Xuan-Rong MO
;
Ling-Ling ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Arthritis, Rheumatoid; metabolism; Cells, Cultured; HSP72 Heat-Shock Proteins; pharmacology; Humans; I-kappa B Proteins; metabolism; Interleukin-6; metabolism; Interleukin-8; metabolism; NF-KappaB Inhibitor alpha; NF-kappa B; metabolism; Signal Transduction; Synovial Membrane; cytology; drug effects; metabolism; Tumor Necrosis Factor-alpha; pharmacology
- From: Chinese Journal of Applied Physiology 2012;28(4):336-339
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of heat shock protein 72 (Hsp72) on the expression of IL-6 and IL-8 and activation of NF-kappaB in synoviocytes from patients suffered from rheumatoid arthritis (RA).
METHODSIL6 and IL8 concentrations in culture supernatants were measured using enzyme-linked immunosorbent assays (ELISA). Nuclear translocation of NF-kappaB and degradation of the inhibitory protein IkappaBalpha were examined using immunohistochemistry and Western blot.
RESULTSHsp72 down-regulated IL-6 and IL-8 production in RA synoviocytes induced by tumor necrosis factor-alpha (TNF-alpha). Hsp72 inhibited nuclear translocation of NF-kappaB and degradation of IkappaBalpha induced by TNF-alpha.
CONCLUSIONHsp72 has an anti-inflammatory effect on RA by down-regulation of IL-6 and IL-8 in synoviocytes, which is mediated through inhibiting the activation of NF-KalphaB signal pathways.
