Effects of CGRP on the E-cadherin expression in human bronchial epithelial cells.
- Author:
Hong-Bo BAI
1
;
Yong-Ping LU
;
Jia-Xi DUAN
;
Yong ZHOU
;
Guo-Ying SUN
;
Cha-Xiang GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; Cadherins; metabolism; Calcitonin Gene-Related Peptide; administration & dosage; pharmacology; Cell Line; Epithelial Cells; drug effects; metabolism; Humans; Ozone; RNA, Messenger; genetics
- From: Chinese Journal of Applied Physiology 2012;28(4):346-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo discuss the effect of calcitonin gene-related peptides (CGRP) on epithelial cadherin (E-cd) expression in human bronchial epithelial cells (HBECs) in vitro.
METHODSThe effect of CGRP on E-cd protein and mRNA expression in both normal and O3-challenged HBECs were determined by immunocytochemistry and RT-PCR. The signal transduction pathways of CGRP were observed by using protein kinase C(PKC) inhibitor (H-7), calmodulin(CaM) inhibitor (W-7) and PKA inhibitor (H-89).
RESULTSCGRP increased E-cd mRNA and protein expressions of normal and O3-challenged HBECs in a dose-dependent manner. CGRP had no effect on cytoplasm E-cd expression. Pre-treatment with H-89, H-7 and W-7, the up-regulatory effect of CGRP on E-cd expression was partly abolished.
CONCLUSIONCGRP increased in cytomembrane E-cd expression of normal and O3-challenged HBECs in a dose-dependent manner. E-cd expression on HBECs was strengthened by CGRP via PKA, PKC and CaM pathways.
