Role of epidermal growth factor receptor expression level in cetuximab cytotoxicity and antibody-dependent cell-mediated cytotoxicity effect against A549 lung cancer cell line.
- Author:
Jin-yu LI
1
;
Shun-chang JIAO
1
;
Guo-qing ZHANG
1
;
Sheng-jie SUN
1
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; pathology; Antibodies, Monoclonal, Humanized; pharmacology; Antineoplastic Agents; pharmacology; Cetuximab; Humans; Immunohistochemistry; Lung Neoplasms; pathology; Receptor, Epidermal Growth Factor; metabolism; Tumor Cells, Cultured
- From: Acta Academiae Medicinae Sinicae 2014;36(2):164-167
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of epidermal growth factor receptor (EGFR) expression level in cetuximab cytotoxicity and antibody-dependent cell-mediated cytotoxicity (ADCC) effect against A549 lung cancer cell line.
METHODSA549 cell line and NKTm cells were used as the target cell and the effector cell, respectively. pEGFR-EGFP plasmids were transfected into A549 cells by nucleofector method. EGFR expression levels were measured by immunohistochemistry. The ADCC activity induced by cetuximab was assessed by cell counting kit-8 assay.
RESULTSA549 cells transfected with pEGFR-EGFP plasmids expressed higher level of EGFR protein on membrane and were more sensitive to ADCC activity mediated by cetuximab (P<0.05). The inhibition rate of A549 cells showed no significant difference between transfection group and wild-type group when treated with cetuximab alone (P> 0.05).
CONCLUSIONEGFR expression level influences the sensitivity of A549 lung cancer cell line to ADCC activity mediated by cetuximab but not to cetuximab alone.
