TXNDC5 mediates serum starvation-induced proliferation inhibition of HeLa cell.
- Author:
Hong-fei ZHANG
1
;
Jie-wen ZHANG
1
;
Li-juan KONG
1
;
Le WANG
1
;
Ning ZHU
1
;
Si-chao GUO
1
;
Chuan QIU
1
;
Xue-jing YAN
1
;
Mei-hong CHEN
1
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; Cell Proliferation; Culture Media; chemistry; Gene Knockdown Techniques; HeLa Cells; Humans; Protein Disulfide-Isomerases; genetics; metabolism; Serum; chemistry
- From: Acta Academiae Medicinae Sinicae 2014;36(5):470-476
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of TXNDC5 in serum starvation-induced proliferation inhibition of HeLa cell.
METHODSTXNDC5 was either over-expressed or knocked down by small interfering RNA (siRNA) in HeLa cells which were then cultured in conventional medium or serum starvation medium. The protein level of TXNDC5 was evaluated by Western blot analysis. The mRNA level of TXNDC5 was measured by quantitative real-time PCR. Cell growth rate was determined by cell proliferation assay kit (MTS method). Cell cycle distribution and apoptosis were detected by flow cytometry.
RESULTSSerum starvation mildly reduced the mRNA level of TXNDC5 (P<0.05), but dramatically increased the protein level of TXNDC5 in HeLa cells. The stability of TXNDC5 mRNA remained unchanged. Cycloheximide abolished the serum starvation-induced up-regulation of TXNDC5 protein. Over-expression of TXNDC5 had no effect on cell proliferation. However, suppression of TXNDC5 attenuated the proliferation inhibition of HeLa cell induced by serum starvation (P<0.05), increased the proportion of cells in S phase (P<0.05), but had no effect on cell apoptosis.
CONCLUSIONTXNDC5 mediates serum starvation-induced proliferation inhibition of HeLa cell.
