Effect of bone morphogenetic protein 7 on differentiation of adipose derived mesenchymal stem cells into brown adipocytes in rats.
- Author:
Long ZHENG
1
;
Jian-Min LIU
1
;
Jun-Xia WANG
1
;
Min-Zhi LI
2
;
Wei-Guang LIAN
1
;
Peng XIE
1
;
Shu-Feng LIU
1
Author Information
- Publication Type:Journal Article
- MeSH: Adipocytes, Brown; physiology; Adipose Tissue; metabolism; Adipose Tissue, Brown; physiology; Animals; Bone Morphogenetic Protein 7; metabolism; Cell Differentiation; physiology; Ion Channels; metabolism; Mesenchymal Stromal Cells; physiology; Mitochondrial Proteins; metabolism; Obesity; metabolism; Rats; Uncoupling Protein 1
- From: Acta Academiae Medicinae Sinicae 2014;36(6):654-659
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo evaluate the effect of bone morphogenetic protein(BMP7)on the differentiation of adipose derived mesenchymal stem cells(AD-MSCs)isolated from different adipose tissues into brown adipocytes in rats.
METHODSPrimary AD-MSCs were isolated from rate interscapular brown adipose tissue(iBAT),inguinal subcutaneous white adipose tissue(sWAT),and epididymal white adipose tissue(eWAT),respectively,and then cultivated in vitro. Differentiation of AD-MSCs into brown adipocytes was induced by BMP7. The characteristics of brown adipocytes were detected by immunofluorescence staining and oil red staining of cells. The expression levels of brown adipocyte-related genes were detected by polymerase chain reaction.
RESULTSAD-MSCs from iBAT and sWAT were differentiated into cluster multilocular cells,which were stained red by oil red "O"staining and showed uncoupling protein 1-positive by immunofluorescent staining method. AD-MSCs from eWAT had a small number of scattered multilocular cells and showed uncoupling protein 1-negative. The results of reverse transcription-polymerase chain reaction showed that the uncoupling protein 1 gene was highly expressed in the iBAT group and sWAT group but was negative in the eWAT group.
CONCLUSIONAD-MSCs isolated from different adipose tissues in rats have different gene expression profiles and differentiation potentials.