Transmission FTIR derivative spectroscopy for estimation of furosemide in raw material and tablet dosage form.
10.1016/j.apsb.2014.06.013
- Author:
Máximo GALLIGNANI
1
;
Rebeca A RONDÓN
1
;
José F OVALLES
2
;
María R BRUNETTO
1
Author Information
1. Laboratorio de Espectroscopia Molecular, Departamento de Química, Facultad de Ciencias, Universidad de Los Andes, Mérida 5101-A, Venezuela.
2. Departamento de Análisis y Control, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida 5101-A, Venezuela.
- Publication Type:Journal Article
- Keywords:
API, active pharmaceutical ingredient;
DMF, N,N-dimethylformamide;
DS, derivative spectroscopy;
Derivative spectroscopy;
FTIR;
FTIR, Fourier transform infrared;
FUR, furosemide or frusemide;
Frusemide;
Furosemide;
HPLC, high performance liquid chromatographic;
MIR, mid infrared;
Pharmaceutical analysis
- From:
Acta Pharmaceutica Sinica B
2014;4(5):376-383
- CountryChina
- Language:English
-
Abstract:
A Fourier transform infrared derivative spectroscopy (FTIR-DS) method has been developed for determining furosemide (FUR) in pharmaceutical solid dosage form. The method involves the extraction of FUR from tablets with N,N-dimethylformamide by sonication and direct measurement in liquid phase mode using a reduced path length cell. In general, the spectra were measured in transmission mode and the equipment was configured to collect a spectrum at 4 cm(-1) resolution and a 13 s collection time (10 scans co-added). The spectra were collected between 1400 cm(-1) and 450 cm(-1). Derivative spectroscopy was used for data processing and quantitative measurement using the peak area of the second order spectrum of the major spectral band found at 1165 cm(-1) (SO2 stretching of FUR) with baseline correction. The method fulfilled most validation requirements in the 2 mg/mL and 20 mg/mL range, with a 0.9998 coefficient of determination obtained by simple calibration model, and a general coefficient of variation <2%. The mean recovery for the proposed assay method resulted within the (100±3)% over the 80%-120% range of the target concentration. The results agree with a pharmacopoeial method and, therefore, could be considered interchangeable.
