Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography-tandem mass spectrometry.
10.1016/j.apsb.2014.05.003
- Author:
Zhongliang ZHANG
1
;
Qiang LI
1
;
Qiufen LI
1
;
Simiao DU
1
;
Yongquan ZHOU
1
;
Chunming LV
1
;
Yan ZHAO
1
;
Yongjun WANG
2
;
Ning ZHANG
1
Author Information
1. Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
2. Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
- Publication Type:Journal Article
- Keywords:
5-O-M, 5-O-methylvisammioside;
AST-III, astragaloside III;
AST-IV, astragaloside IV;
Active components;
BER, berberine;
CA, cholic acid;
CCS, calycosin;
CCSG, calycosin-7-O-β-d-glucoside;
CE, collision energy;
CSR, cervical spondylosis radiculopathy;
DAI, daidzein;
FAN, fangchinoline;
FOR, formononetin;
GA, gallic acid;
ONO, ononin;
PAL, palmatine;
QSP, Qi She Pill;
Qi She Pill (QSP);
RA, rheumatoid arthritis;
SEA, senkyunolide A;
SEI, senkyunolide I;
SIN, sinomenine;
SRM, selective reaction monitoring;
TCM, traditional Chinese medicine;
TET, (+)-tetrandrine;
THP, tetrahydropalmatine;
THPB, tetrahydroepiberberine;
Traditional Chinese medicine;
UHPLC–MS/MS
- From:
Acta Pharmaceutica Sinica B
2014;4(5):384-393
- CountryChina
- Language:English
-
Abstract:
Qi She Pill (QSP) is a traditional Chinese medicine (TCM) prescription that has been used in treating cervical spondylosis radiculopathy for many years. In this study, a simple and sensitive method using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP. We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol. The correlation coefficients of all calibration curves were greater than 0.99. Recoveries measured at three concentration levels varied from 95.43% to 102.35%. Relative standard deviations of intra- and inter-day precisions were less than 4.45%. After successfully validating our method, we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components.
