Design, synthesis and evaluation of genistein-polyamine conjugates as multi-functional anti-Alzheimer agents.
10.1016/j.apsb.2014.12.008
- Author:
Xin ZHANG
1
;
Jiang WANG
1
;
Chen HONG
1
;
Wen LUO
1
;
Chaojie WANG
1
Author Information
1. Key Laboratory of Natural Medicine and Immuno-Engineering, Henan University, Kaifeng 475004, China.
- Publication Type:Journal Article
- Keywords:
Acetylcholinesterase;
Alzheimer׳s disease;
Genistein;
Inhibition;
Metal-chelating;
Molecular modeling;
Polyamine;
Rivastigmine
- From:
Acta Pharmaceutica Sinica B
2015;5(1):67-73
- CountryChina
- Language:English
-
Abstract:
A series of genistein-polyamine conjugates (4a-4h) were designed, synthesized and evaluated as multi-functional anti-Alzheimer agents. The results showed that these compounds had significant cholinesterases (ChEs) inhibitory activity. Compound 4b exhibited the strongest inhibition to acetylcholinesterase (AChE) with an IC50 value of 2.75 μmol/L, which was better than that of rivastigmine (5.60 μmol/L). Lineweaver-Burk plot and molecular modeling study showed that compound 4b targeted both the catalytic active site (CAS) and the peripheral anionic site (PAS) of AChE. Besides, compound 4b showed potent metal-chelating ability. In addition, it was found that 4a-4h did not affect HepG-2 cell viability at the concentration of 10 μmol/L.
