Differentiation of rat neural stem cells and its relationship with environment.
- Author:
Yi-Hua AN
1
;
Hong-Yun WANG
;
Zhi-Xian GAO
;
Zhong-Cheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Astrocytes; cytology; metabolism; Caudate Nucleus; metabolism; pathology; Cell Differentiation; physiology; Cell Movement; physiology; Cells, Cultured; Cerebral Hemorrhage; pathology; surgery; Coculture Techniques; Fluorescent Antibody Technique; Galactosylceramides; metabolism; Glial Fibrillary Acidic Protein; metabolism; Microscopy, Phase-Contrast; Microtubule-Associated Proteins; metabolism; Oligodendroglia; cytology; metabolism; Rats; Rats, Sprague-Dawley; Schwann Cells; cytology; Sciatic Nerve; cytology; Stem Cell Transplantation; Stem Cells; cytology
- From: Biomedical and Environmental Sciences 2004;17(1):1-7
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the differentiation fates of rat neural stem cells (NSCs) in different environmental conditions.
METHODSNSCs derived from 16-day-old rat embryo were proliferated in vitro and implanted into the brain of rats with intra-cerebral hemorrhage. At the same time some NSCs were co-cultured in vitro with Schwann cells derived from newborn rats. MAP-2, GFAP and GalC (which are the specific markers of neural cells, astrocytes and oligodendrocytes respectively), BrdU and beta-tubulin were detected by immunohistochemical and immunofluorescent methods.
RESULTSBrdU positive cells that were implanted into the brain distributed around the hemorrhagic area. The majority of them were GFAP positive astrocytes while a few of them were beta-tubulin positive neural cells or GalC positive oligodendrocytes. After being co-cultured with Schwann cells in vitro, NSCs are predominately shown beta-tubulin and MAP-2 positive, and only a minority of them were GFAP or GalC positive.
CONCLUSIONSThe hemorrhagic environment in vivo induces NSCs to differentiate mainly into astrocytes while co-culture with Schwann cells in vitro induce the majority of NSCs to differentiate into neural cells.
