ROS-related enzyme expressions in endothelial cells regulated by tea polyphenols.
- Author:
Chen-Jiang YING
1
;
Xiu-Fa SUN
;
Shu-Lin ZHANG
;
Xi-Ping ZHANG
;
Li-Mei MAO
;
Xue-Zhi ZUO
;
Ping YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Camellia sinensis; chemistry; Carotid Arteries; cytology; Catalase; biosynthesis; Cattle; Cells, Cultured; Down-Regulation; Endothelial Cells; drug effects; enzymology; metabolism; Flavonoids; isolation & purification; pharmacology; Membrane Transport Proteins; biosynthesis; NADPH Dehydrogenase; biosynthesis; NADPH Oxidases; Phenols; isolation & purification; pharmacology; Phosphoproteins; biosynthesis; Polyphenols; Reactive Oxygen Species; metabolism; Superoxide Dismutase; biosynthesis; Superoxide Dismutase-1; Up-Regulation
- From: Biomedical and Environmental Sciences 2004;17(1):33-39
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEElevation of reactive oxygen species (ROS), especially the level of superoxide is a key event in many forms of cardiovascular diseases. To study the mechanism of tea polyphenols against cardiovascular diseases, we observed the expressions of ROS-related enzymes in endothelial cells.
METHODSTea polyphenols were co-incubated with bovine carotid artery endothelial cells (BCAECs) in vitro and intracellular NADPH oxidase subunits p22phox and p67phox, SOD-1, and catalase protein were detected using Western blot method.
RESULTSTea polyphenols of 0.4 microg/mL and 4.0 microg/mL (from either green tea or black tea) down-regulated NADPH oxidase p22phox and p67phox expressions in a dose-negative manner (P < 0.05), and up-regulated the expressions of catalase (P < 0.05).
CONCLUSIONSTea polyphenols regulate the enzymes involved in ROS production and elimination in endothelial cells, and may be beneficial to the prevention of endothelial cell dysfunction and the development of cardiovascular diseases.
