Effects of AGEs on oxidation stress and antioxidation abilities in cultured astrocytes.
- Author:
Jian-Ming JIANG
1
;
Zhen WANG
;
Dian-Dong LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Astrocytes; drug effects; enzymology; metabolism; Cattle; Cells, Cultured; Cerebral Cortex; cytology; Glutathione; metabolism; Glycation End Products, Advanced; pharmacology; Malondialdehyde; metabolism; Monoamine Oxidase; metabolism; Nitric Oxide; metabolism; Oxidative Stress; drug effects; Rats; Rats, Wistar; Serum Albumin, Bovine; pharmacology; Superoxide Dismutase; metabolism
- From: Biomedical and Environmental Sciences 2004;17(1):79-86
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate whether two kinds of in vitro prepared advanced glycation end products (AGEs), Glu-BSA and Gal-BSA, could change oxidation stress and anti-oxidation abilities in astrocytes, and thus might contribute to brain injury.
METHODSChanges of GSH, MDA, SOD, MAO-B, nitric oxide were measured after AGEs treatment.
RESULTSBoth 0.1 g/L Glu-BSA and Gal-BSA could slightly decrease GSH level, while 1 g/L of them significantly decreased GSH level by 35% and 43% respectively. The MDA levels of both 1 g/L AGEs treated groups (306 +/- 13 and 346 +/- 22) were higher than that of the normal group (189 +/- 18), which could be inhibited by free radical scavenger NAC. The SOD activities of both 1 g/L AGEs treated groups (67.0 +/- 5.2 and 74.0 +/- 11.0) were lower than that of the normal group (85.2 +/- 8.0). Both 0.1 g/L AGEs could slightly increase the activity of MAO-B, while 1 g/L of them could increase MAO-B activity by 1.5 and 1.7 folds respectively. Both AGEs stimulation could produce NO level by 1.7 and 2 folds respectively.
CONCLUSIONEnhanced levels of astrocytic oxidation stress and decrease of antioxidation abilities may contribute to, at least partially, the detrimental effects of AGEs in neuronal disorders and aging brain.
