Effects of fluoride on lipid peroxidation, DNA damage and apoptosis in human embryo hepatocytes.
- Author:
Ai-Guo WANG
1
;
Tao XIA
;
Qi-Long CHU
;
Ming ZHANG
;
Fang LIU
;
Xue-Min CHEN
;
Ke-Di YANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; drug effects; Cells, Cultured; Comet Assay; DNA; drug effects; DNA Damage; Dose-Response Relationship, Drug; Glutathione; analysis; metabolism; Hepatocytes; drug effects; metabolism; pathology; Humans; Lipid Peroxidation; Lipid Peroxides; analysis; metabolism; Liver; drug effects; embryology; pathology; Proteins; analysis; Sodium Fluoride; pharmacology
- From: Biomedical and Environmental Sciences 2004;17(2):217-222
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of fluoride on lipid peroxidation, DNA damage and apoptosis in human embryo hepatocyte L-02 cells.
METHODSLipid peroxide (LPO) level, reduced glutathione (GSH) content, DNA damage, apoptosis, and cell cycle analysis were measured after in vitro cultured L-02 cells were exposed to sodium fluoride at different doses (40 microg/mL, 80 microg/mL, and 160 microg/mL) for 24 hours.
RESULTSFluoride caused an increase of LPO levels and a decrease of GSH content in L-02 cells. There appeared to be an obvious dose-effect relationship between the fluoride concentration and the observed changes. Fluoride also caused DNA damage and apoptosis and increased the cell number in S phase of cell cycle in the cells tested. There was a statistically significant difference in DNA damage and apoptosis when comparing the high dose of fluoride treated cells with the low dose of fluoride treated cells.
CONCLUSIONFluoride can cause lipid peroxidation, DNA damage, and apoptosis in the L-02 cell experimental model and there is a significant positive correlation between fluoride concentration and these pathological changes.
