Identification of a new peptide deformylase gene from enterococcus faecium and establishment of a new screening model targeted on PDF for novel antibiotics.
- Author:
Xian-Bing TANG
1
;
Shu-Yi SI
;
Yue-Qin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amidohydrolases; antagonists & inhibitors; genetics; isolation & purification; Amino Acid Sequence; Anti-Bacterial Agents; therapeutic use; Cloning, Molecular; Drug Evaluation, Preclinical; Enterococcus faecium; enzymology; genetics; Fluorescamine; Fluorescence; Hydrogen-Ion Concentration; Indicators and Reagents; Kinetics; Models, Molecular; Molecular Sequence Data; Temperature
- From: Biomedical and Environmental Sciences 2004;17(3):350-358
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo identify a new peptide deformylase (PDF) gene (Genebank Accession AY238515) from Enterococcus faecium and to establish a new screening model targeted on PDF.
METHODSA new PDF gene was identified by BLAST analysis and PCR and was subsequently over-expressed in the prokaryotic expression host E. coli B121(DE3). Over-expressed protein was purified for enzymatic assay by metal affinity chromatography and a new screening model was established for novel antibiotics.
RESULTA new PDF gene of Enterococcus faecium was identified successfully. Ten positive samples were picked up from 8000 compound library and the microbial fermentation broth samples.
CONCLUSIONA new PDF of gene Enterococcus faecium was first identified and the model had a high efficacy. Positive samples screened may be antibacterial agents of broad spectrum.
