8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage induced by environmental tobacco side-stream smoke and its mechanism.
- Author:
Zhu-Ge XI
1
;
Fu-Huan CHAO
;
Dan-Feng YANG
;
Hua-Shan ZHANG
;
Wei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers; analysis; Cattle; DNA; drug effects; metabolism; DNA Adducts; analysis; DNA Damage; Deoxyguanosine; analogs & derivatives; analysis; Female; Lung; chemistry; metabolism; Metals, Heavy; analysis; Organic Chemicals; analysis; Oxidation-Reduction; Rats; Tobacco Smoke Pollution; adverse effects; analysis
- From: Biomedical and Environmental Sciences 2005;18(1):43-47
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the genotoxicity effect of environmental tobacco side-stream smokes (ETSS) on oxidative DNA damage and its molecular mechanism.
METHODSDNA adduct 8-hydroxydeoxyguanosine (8-OHdG) was used as a biomarker of oxidative DNA damage. The level of 8-OHdG in DNA exposed to ETSS was detected by high performance liquid chromatography with electrochemical detection. Organic and inorganic components in ETSS were analyzed by gas chromatography-mass spectrum and atomic absorption spectrum respectively.
RESULTSParticle matters (PMs) and volatile organic compounds (VOCs) in ETSS could directly induce oxidative DNA damage and formation of 8-OHdG. There were 123 and 84 kinds of organic components in PMs and VOCs respectively, and 7 kinds of inorganic components in ETSS. Some components, especially quinones and polyphenols in ETSS, could produce free radicals in vitro by auto-oxidation without any biological activity systems, and with the catalytic reaction of metals, the DNA adduct 8-OHdG was produced.
CONCLUSIONETSS have biological oxidative effect on DNA in vitro and in vivo, and expressed direct genotoxicity. 8-OHdG is a valuable biomarker of oxidative DNA damage.
