Effects of formaldehyde inhalation on lung of rats.
- Author:
Yu-Hua YANG
1
;
Zhu-Ge XI
;
Fu-Huan CHAO
;
Dan-Feng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Inhalation; Animals; Databases, Protein; Electrophoresis, Gel, Two-Dimensional; Female; Formaldehyde; toxicity; Lung; drug effects; metabolism; Proteins; metabolism; Proteomics; Rats; Rats, Wistar; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Biomedical and Environmental Sciences 2005;18(3):164-168
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo analyze protein changes in the lung of Wistar rats exposed to gaseous formaldehyde (FA) at 32-37 mg/m3 for 4 h/day for 15 days using proteomics technique.
METHODSLung samples were solubilized and separated by two-dimensional electrophoresis (2-DE), and gel patterns were scanned and analyzed for detection of differently expressed protein spots. These protein spots were identified by MALDI-TOF-MS and NCBInr protein database searching.
RESULTSFour proteins were altered significantly in 32-37 mg/m3 FA group, with 3 proteins up-regulated, 1 protein down-regulated. The 4 proteins were identified as aldose reductase, LIM protein, glyceraldehyde-3-phosphate dehydrogenase, and chloride intracellular channel 3.
CONCLUSIONThe four proteins are related to cell proliferation induced by FA and defense reaction of anti-oxidation. Proteomics is a powerful tool in research of environmental health, and has prospects in search for protein markers for disease diagnosis and monitoring.
