Molecular epidemiology of Norovirus in outbreaks of gastroenteritis in Zhejiang from 2006 to 2007
10.3760/cma.j.issn.0254-6450.2009.02.012
- VernacularTitle:浙江省2006-2007年暴发性胃肠炎中诺如病毒的分子流行病学分析
- Author:
Li-Ming GONG
1
;
Qiong GE
;
Yi-Yu LU
;
Yan-Jun ZHANG
;
Ju-Ying YAN
;
Min ZHOU
;
Zhao YU
Author Information
1. 浙江省疾病预防控制中心
- Keywords:
Norovirus;
Outbreaks ofgastroenteritis;
Molecular epidemiology
- From:
Chinese Journal of Epidemiology
2009;30(2):147-150
- CountryChina
- Language:Chinese
-
Abstract:
Objecfive To study the molecular epidemioiogical characteristics of Norovirus gastroenteritis outbreaks in Zhejiang.Methods During January 2006 and December 2007.fecal specimens of patients collected from outbreaks of acute viml gastroenteritis were tcsted for Norovirus.Epidemiological data were also collected.Noroviruses were detected by a reverse transcription polymerase chain reaction(RT-PeR)and Real.time RT-PCR.Some positive samples were randomly selected and Rrr-PCR products were sequenced.Comparing to the nucleotide sequences of norovirus genotype Ⅰ,Ⅱ reference strains from GenBank,sequence analysis was undertaken based on partial sequence of RNA dependent RNA polymerase(RdRp)and capsid protein(VPI)gene.Results 5 Outbreaks of viral gastroenteritis caused bv Norovirus were reported.A total of 63 stools were obtained from cases with acute gastroenteritis.Noroviruses alone were detected in 45 cases and the illness appeared in autumn.Phylogenetic analysis revealed that Norovirus belonged to G Ⅱ/G Ⅱ 4 type.The strains isolated from Zhejiang were almost identical on G Ⅱ/4 variants that causing epidemics in Beijing and in the Netherlands with the homology of 99.7%and 98.5%-99.O%respectively.Phylogenetic analysis revealed that the isolates were located at the same branch as the norovirus G Ⅱ/4 variants found in Beijing and Netherlands.Conclusion Norovirus iS a major cause of outbreaks of viral gastroenterifis in Zhejiang province.GenogroupⅡ/4 variants viruses were the prevalent strains.
