Establishment and preliminary application of screening methods for Y chromosome microdeletions in male infertility patients.
- Author:
Cong-yi YU
1
;
Guang-lun ZHUANG
;
Can-quan ZHOU
;
Zong-he YAN
;
Wei LI
;
Hua GAO
;
De-rong RUI
Author Information
- Publication Type:Journal Article
- MeSH: Chromosome Deletion; Chromosomes, Human, Y; genetics; Female; Genetic Testing; methods; Humans; Infertility, Male; diagnosis; genetics; Male; Polymerase Chain Reaction
- From: Chinese Journal of Medical Genetics 2003;20(4):357-359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a multiplex PCR protocol, which could be suitable for routine screening of microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.
METHODSFive multiplex sets were established. Eighty-seven azoospermic and oligozoospermic patients undergoing intracytoplasmic sperm injection (ICSI) in the in vitro fertilization (IVF) center and 30 azoospermic men undergoing testicular biopsy in the clinic of Urology Surgery were screened for microdeletions of Y chromosome.
RESULTSA total of 19 (16.2%) cases of microdeletions were found in 117 azoospermic and oligozoospermic patients by screening of Y chromosome microdeletions. Of these, 11 cases (18.0%) were found in 61 oligozoospermic patients, and 8 cases (14.3%) were found in 56 azoospermic patients.
CONCLUSIONThe multiplex PCR protocol presented in this study is an easy-to-do and reliable method for detecting microdeletions on the Y chromosome. Routine screening of microdeletions on the Y chromosome for azoospermic and oligozoospermic patients is essential.
