Evaluation of fetal DNA in maternal plasma for prenatal diagnosis.
- Author:
Yin ZHAO
1
;
Li ZOU
Author Information
- Publication Type:Journal Article
- MeSH: DNA; blood; genetics; Female; Fetus; metabolism; Humans; Pregnancy; Prenatal Diagnosis; methods; Sex Determination Processes
- From: Chinese Journal of Medical Genetics 2003;20(4):360-362
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the application of fetal DNA in maternal plasma for uninvasive prenatal diagnosis.
METHODSThe DNA template was extracted by hydroxybenzene-chloroform from 44 maternal (7-41 weeks) plasma. A Y-chromosome-specific repeat sequence-DYZ1 gene (149 bp) was chosen to be amplified by PCR.
RESULTSThe fragment was identified in all the plasma of male bearing pregnant women. The diagnostic accordance rate was 100.00%; two of the 22 female bearing pregnant women had false positive results. Among the 44 pregnant women, the diagnostic accordance rate was 88.89% at early pregnant stage, 100.00% at medium pregnant stage, and 96.55% at late stage respectively. The final accuracy of 95.45% was obtained in all cases.
CONCLUSIONBy means of hydroxybenzene-chloroform extraction the authors of this article promoted the concentration and purity of the DNA template, and diagnosed more accurately. The results showed that free fetal DNA in the maternal plasma could be regarded as the gene resource for uninvasive prenatal diagnosis.
