Detecting MTHFR gene mutation with a new approach: molecular beacons.
- Author:
Bao-qin JIANG
1
;
Guo-ming ZHU
;
Jin-hua YU
;
Qing-qiu BAO
;
Mei XU
Author Information
- Publication Type:Journal Article
- MeSH: DNA Mutational Analysis; methods; Fluorescent Dyes; chemistry; Genotype; Humans; Methylenetetrahydrofolate Reductase (NADPH2); genetics; Oligonucleotide Probes; chemistry; genetics; Point Mutation; Sensitivity and Specificity
- From: Chinese Journal of Medical Genetics 2003;20(5):436-437
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the methylenetetrahydrofolate reductase(MTHFR) gene C677T mutation with molecular beacon technique and assess the revant applicability.
METHODSA total of 228 samples were analyzed using molecular beacons which are oligonucleotide probes to become fluorescent upon hybridization. Wild-type molecular beacon and mutant beacon were designed to detect the genotypes of MTHFR gene.
RESULTSAnalysis of the 228 samples indicated that there were three genotypes including 41 homozygous mutants, 113 heterozygous individuals and 74 wild-type individuals. Every sample was identified clearly.
CONCLUSIONThe present method, a closed-tube PCR/hybridization assay, is a simple, high-throughput and fast procedure that is fully automated for detecting gene mutation.
