Relationship of interleukin-1B and interleukin-1 receptor antagonist gene polymorphisms with chronic hepatitis B.
- Author:
Ping-an ZHANG
1
;
Yan LI
;
Pu XU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; Female; Genotype; Hepatitis B, Chronic; genetics; Humans; Interleukin 1 Receptor Antagonist Protein; Interleukin-1; genetics; Male; Middle Aged; Polymorphism, Genetic; Sialoglycoproteins; genetics
- From: Chinese Journal of Medical Genetics 2003;20(6):508-511
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the polymorphisms of interleukin-1B (IL-1B) promoter region -511C/T and interleukin-1 receptor antagonist (RN) gene, the relationship between the genotype of IL-1B and IL-1RN, and the susceptibility to chronic hepatitis B in Chinese population.
METHODSGenomic DNA was extracted from the peripheral blood of 190 patients with chronic hepatitis B and 249 normal controls and then was subjected to PCR amplification. The PCR product was digested by restriction endonuclease Ava I. The product of digestion was subjected to 2% gel-electrophoresis and ethidium bromide staining.
RESULTSThe IL-1B -511C allele was detected in 0.50 of normal controls and 0.48 of patients, while the IL-1B -511T allele was detected in 0.50 of normal controls and 0.52 of patients. The frequency of CC genotype was 0.26 (65/249) and in normal controls 0.24 (45/190) in patients. The frequency of CT genotype was 0.47 (118/249) in normal controls and 0.49 (94/190) in patients. The frequency of TT genotype was 0.27 (66/249) in normal controls and 0.27 (51/190) in patients. The HBV-DNA copies in chronic hepatitis B with CC genotype were significantly decreased, compared with controls (P<0.05). Only four (1/1, 1/2, 2/2 and 1/4) of the five kinds of polymorphism of IL-1RN were found in this study. The frequencies of 1/1, 1/2, 2/2 and 1/4 were 0.88, 0.09, 0.01 and 0.02 in chronic hepatitis B patients respectively, while in controls were 0.81, 0.16, 0.01 and 0.02. The IL-1 RN*1 allele was detected in 0.94 of chronic hepatitis B patients and in 0.90 of controls, while the IL-1RN*2 allele was detected in 0.05 of patients and in 0.09 of normal controls. The frequencies of 1/2 genotype and IL-1RN*2 allele were lower in chronic hepatitis B than in controls (P<0.05).
CONCLUSIONThe polymorphisms of the promoter region -511C/T of IL-1B gene and IL-1RN intron 2 gene are associated with the development of chronic hepatitis B. The people with IL-1RN*2 allele may be protected against HBV infection, and the IL-1B -511 CC genotype may be linked to HBV-DNA copy.
