Study on exons 3 and 4 of alpha-synuclein gene in Chinese familial Parkinson disease patients.
- Author:
Xi CHEN
1
;
Yue-ting LIU
;
Jin-ru LI
;
Ling CHEN
;
Yan-ming XU
;
Yong-hui PAN
;
Xiao-hong MENG
;
Shi-hui XING
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Exons; Female; Heteroduplex Analysis; Humans; Male; Middle Aged; Mutation; Nerve Tissue Proteins; genetics; Parkinson Disease; genetics; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Synucleins; alpha-Synuclein
- From: Chinese Journal of Medical Genetics 2003;20(6):536-538
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship of Chinese familial Parkinson disease with alpha-synuclein gene.
METHODSPolymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex analysis(PCR-HA) were employed to detect the abnormal mobilization in the familial Parkinson disease and sporadic Parkinson disease patients, then it was verified by gene sequencing.
RESULTSNo mutation was found in alpha-synuclein gene exons 3 and 4 by PCR-SSCP together with PCR-HA. An inserted c and an inserted t were found in intron 4, position 23 and position 67 respectively.
CONCLUSION(1) Exons 3 and 4 of alpha-synuclein gene are not the mutational hot spots of Chinese familial Parkinson disease. (2) Two polymorphisms were found in intron 4 of alpha-synuclein gene. They are 23 ins c and 67 ins t.
