The relationship between IL-13 gene polymorphism and the levels of serum IL-13 and serum eosinophil cation protein in asthmatic children.
- Author:
Hai-ping SUN
1
;
Jie-qing CHEN
;
Xi-rong GUO
;
Rong-hua CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Asthma; blood; genetics; Blood Proteins; Child; Child, Preschool; Eosinophil Granule Proteins; Female; Genotype; Humans; Infant; Interleukin-13; blood; genetics; Male; Polymorphism, Genetic; Ribonucleases; blood
- From: Chinese Journal of Medical Genetics 2003;20(6):547-548
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between IL-3 gene polymorphism and the levels of serum IL-3 and eosinophil cation protein (ECP) for understanding the role of IL-3 gene polymorphism in the mechanism of childhood asthma.
METHODSThe method of restriction fragment length polymorphism (RFLP) was adopted in detecting +1923 site polymorphism of IL-13 gene in intron 3 region, ELISA was employed in detecting the level of serum IL-13, and fluorescent enzyme-linked immunoassay was used to detect the level of serum ECP.
RESULTSThe frequency distribution of TT, TC genotypes of IL-13 Intron 3+1923 site in asthmatic children was higher than that of CC genotype in normal control (P<0.05), and the levels of serum IL-13 and ECP of TT, TC genotypes were significantly higher than those of CC genotype respectively (P<0.01).
CONCLUSIONThe close relationship of IL-3 gene polymorphism with the levels of serum IL-13 and ECP suggests that IL-3 gene polymorphism may play an important role in the mechanism of childhood asthma.
