Study on the relationship of MTHFR polymorphisms with unexplained recurrent spontaneous abortion.
- Author:
Xiao-mei LI
1
;
You-zhong ZHANG
;
Yan-xue XU
;
Sen JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Abortion, Habitual; blood; enzymology; genetics; Adult; Alleles; China; DNA Mutational Analysis; methods; Female; Folic Acid; blood; Gene Frequency; Genotype; Homocysteine; blood; Humans; Methylenetetrahydrofolate Reductase (NADPH2); genetics; Point Mutation; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Vitamin B 12; blood
- From: Chinese Journal of Medical Genetics 2004;21(1):39-42
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the relationship of methylenetetrahydrofolate reductase (MTHFR) C677T genotypes to unexplained recurrent spontaneous abortion (URSA).
METHODSThis study included two groups:57 currently non-pregnant women with a history of URSA (URSA group), and 50 currently non-pregnant women with a history of having given birth to at least one live baby and without any history of spontaneous abortion, still-born fetus, placental thrombosis and intrauterine growth retardation(IUGR)(control group). The fasting serum-Hcy was measured with high pressure liquid chromatography. Folic acid and vitamin B(12) were detected by radioimmune assay; antiphospholipid antibody (ACA) was detected by ELISA. MTHFR C677T gene polymorphisms were detected by the technique of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
RESULTSC/C genotype in URSA group was significantly lower than that in control group, the total mutant T allele frequency was significantly higher than that in control group. There was no significant difference in respect of "age, rural area/city, period, primary/secondary abortion" between the genotype distributions of MTHFR C677T. The T/T genotype and C/T+T/T genotypes frequencies for "abortion times>or=3" were higher than those for "abortion time <3".
CONCLUSIONMTHFR C677T gene polymorphism is a genetic risk factor for URSA.
