The detection of microsatellite instability by ion-pair reversed-phase high performance liquid chromatography.
- Author:
Xiao-rong LIU
1
;
Ya-ping WANG
;
Jun-ni ZHANG
;
Jin-tian LI
;
Xiao-mei ZHANG
;
Ming ZHU
;
Xiang-nian SHAN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Chromatography, High Pressure Liquid; methods; Colonic Neoplasms; genetics; pathology; Colorectal Neoplasms, Hereditary Nonpolyposis; genetics; pathology; DNA, Neoplasm; analysis; genetics; Humans; Loss of Heterozygosity; Microsatellite Repeats; genetics; Middle Aged; Rectal Neoplasms; genetics; pathology; Reproducibility of Results; Sensitivity and Specificity
- From: Chinese Journal of Medical Genetics 2004;21(1):56-60
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo set up a sensitive and stable technique which has high throughout to detect the instability of microsatellite DNA.
METHODSGenomic DNA extracted from the cancer tissues and their normal tissues were subjected to microsatellite instability(MSI) analysis on five of DNA markers in 115 sporadic colorectal cancers by means of PCR and ion-pair reversed-phase high performance liquid chromatography. Genomic DNA extracted from lymphocytes in blood of 20 normal persons were analysed and used as the standard control.
RESULTSSeventeen (14.8%) MSI-H and 23(20.0%) MSI-L were found in 115 sporadic colorectal cancers. The rates of MSI in the young patients and old patients were much higher than that in the middle-age patients (P<0.05). And the rate of MSI in low differentiation group was also much higher than that in high or middle differentiation groups (P<0.05).
CONCLUSIONThe method the authors developed is a sensitive and accurate technique to detect MSI and has a high throughput.
