Sequence polymorphisms of the mitochondrial DNA control region in 100 Chengdu Hans in China.
- Author:
Wei-bo LIANG
1
;
Mei-li LU
;
Yi JIA
;
Bin ZHOU
;
Kuan-lin LIU
;
Jing-hui CHEN
;
Miao LIAO
;
Mei-yun WU
;
Lin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; China; DNA, Mitochondrial; chemistry; Genetics, Population; Humans; Molecular Sequence Data; Mutation; Polymorphism, Genetic
- From: Chinese Journal of Medical Genetics 2004;21(2):144-148
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genetic polymorphisms of the mitochondrial DNA (mtDNA) control region in Chengdu Han population.
METHODSSequence polymorphisms of the mtDNA control region, hypervariable regions I and II from 100 unrelated Chinese Hans were determined by PCR and direct sequencing.
RESULTSSequences of 404 nucleotides for hypervariable region I and 379 nucleotides for region II were obtained. Ninety-two and fifty variable sites were revealed in region I and region II respectively as compared to the reference sequence, and a total of 97 different genetic patterns from both the regions I and II were determined. The probability of identity was estimated at 1.84% for region I, 1.94% for region II, and 1.18% for both the regions.
CONCLUSIONThese results suggest that sequence polymorphism of mtDNA control region would be very useful in forensic practice as a marker for individual identification.
