Cloning, identification and preliminary functional analysis of odorant receptor 7 gene of Aedes albopictus.
- Author:
Ning ZHOU
1
;
Xiaoming WANG
;
Yuhua DENG
;
Hongmei LIU
;
Tong LIU
;
Xiaoguang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Aedes; genetics; Animals; Cloning, Molecular; Female; Gene Expression; Genes, Insect; HEK293 Cells; Humans; Larva; Pupa; Receptors, Odorant; genetics
- From: Journal of Southern Medical University 2014;34(10):1420-1425
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone and identify olfactory receptor odorant receptor 7 (OR7) gene of Aedes albopictus and analyze its expression profile and calcium regulation function.
METHODRT-PCR was used to amplify the olfactory receptor OR7 gene of Ae. albopictus and OR7 expression was detected in different tissues and organs. The coding sequence of OR7 gene was cloned in eukaryotic expression vector pME18s, which was then transfected into HEK293 cells. The calcium callback function in response to odor molecule stimulation was analyzed by calcium imaging technique.
RESULTSThe OR7 gene of Ae. albopictus was cloned and sequence analysis showed that its coding region was 1395 bp. RT-PCR detected OR7 expression in the larvae, pupae and adult mosquitoes, especially in female mosquitos. Preliminary analysis of calcium callback function demonstrated the specific regulation of calcium absorption by OR7 in response to odor molecule stimulation.
CONCLUSIONThe OR7 gene of Ae. albopictus has been cloned successfully. OR7 is highly expressed in female mosquitos and is capable of specific recognition of the odor molecules.
