Effect of Biejiajian Pills on Wnt signal pathway molecules β-catenin and GSK-3β and the target genes CD44v6 and VEGF in hepatocellular carcinoma cells.
- Author:
Haitao SUN
1
;
Songqi HE
;
Bin WEN
;
Wenyan JIA
;
Eryan FAN
;
Yan ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Hepatocellular; metabolism; pathology; Down-Regulation; Drugs, Chinese Herbal; pharmacology; Glycogen Synthase Kinase 3; metabolism; Glycogen Synthase Kinase 3 beta; Hep G2 Cells; drug effects; Humans; Hyaluronan Receptors; metabolism; Liver Neoplasms; metabolism; pathology; Phosphorylation; Rats; Vascular Endothelial Growth Factor A; metabolism; Wnt Signaling Pathway; drug effects; beta Catenin; metabolism
- From: Journal of Southern Medical University 2014;34(10):1454-1458
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Biejiajian Pills on the expressions of the signal molecules and target genes of Wnt signal pathway in HepG2 cells and explore the mechanisms by which Biejiajian pills suppress the invasiveness of hepatocellular carcinoma.
METHODSHepG2 cells were cultured for 48 h in the presence of serum collected from rats fed with Biejiajian Pills. The expressions of β-catenin, GSK-3β and P-GSK-3β in the cultured cells were assessed by Western blotting and the expressions of CD44v6 and VEGF were detected using immunohistochemistry.
RESULTSHepG2 cells cultured with the serum of rats fed with Biejiajian Pills showed lowered expressions of β-catenin protein both in the cytoplasm and the nuclei with also inhibition of phosphorylation of GSK-3β and reduced expression of CD44v6 and VEGF.
CONCLUSIONBiejiajian Pills can significantly reduce the expression of β-catenin by decreasing the phosphorylation of GSK-3β and blocking the Wnt/β-catenin signaling pathway to cause down-regulation of the target genes CD44v6 and VEGF, which may be one of the molecular mechanisms by which Biejiajian Pills suppress the proliferation and invasiveness of hepatocellular carcinoma.
